Therapeutic intervention with chicken egg white ovomacroglobulin and a new quinolone on experimental Pseudomonas keratitis.

Abstract

BACKGROUND:

Chicken egg white ovomacroglobulin (ovoM) is a potent protease inhibitor with broad-spectrum activity against various proteases. The combined effects of ovoM and the new quinolone, ofloxacin (OFLX) on experimental Pseudomonas aeruginosa keratitis were investigated.

METHODS:

The in vitro inhibitory effects of ovoM on protease activity in culture fluid of clinically isolated P. aeruginosa and on activity of human neutrophil elastase and cathepsin G were assayed using azo-casein as substrate. Albino rabbits received intrastromal injection of the isolated Pseudomonas strain (1 x 10(5) colony-forming units). At 16 h after inoculation, three treatment groups--0.1% ovoM alone, 0.3% OFLX alone, and a combination of both--and a non-treatment control group were tested.

RESULTS:

Protease activity in the culture solution and human neutrophil elastase was inhibited by ovoM, whereas cathepsin G was not inhibited effectively. In vivo additive therapeutic effects of ovoM and OFLX were observed at 96 h (P < 0.05 compared with OFLX alone).

CONCLUSION:

The results indicate that inhibition of proteolytic activity with ovoM is useful in preventing stromal degradation in P. aeruginosa keratitis.

• Authors: S Miyagawa, R Kamata, K Matsumoto, R Okamura, H Maeda
• Journal: Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie (Graefes Arch Clin Exp Ophthalmol) Vol. 232 Issue 8 Pg. 488-93 (Aug 1994) ISSN: 0721-832X [Print] Germany
• PMID: 7926884 (Publication Type: Journal Article)

Chemical References

• Egg Proteins, Dietary
• Macroglobulins
• alpha-Macroglobulins
• ovomacroglobulin
• Ofloxacin
• Cathepsins
• Serine Endopeptidases
• CTSG protein, human
• Cathepsin G
• Pancreatic Elastase
• Leukocyte Elastase

Topics

• Animals
• Cathepsin G
• Cathepsins (metabolism)
• Egg Proteins, Dietary (therapeutic use)
• Eye Infections, Bacterial (drug therapy)
• Keratitis (drug therapy, microbiology)
• Leukocyte Elastase (metabolism)
• Macroglobulins (therapeutic use)
• Male
• Ofloxacin (therapeutic use)
• Pancreatic Elastase (metabolism)
• Pseudomonas (enzymology)
• Pseudomonas Infections (drug therapy)
• Rabbits
• Serine Endopeptidases
• alpha-Macroglobulins