Cloning and activation of the Syrian hamster neu proto-oncogene.

Abstract

Point mutations of the Syrian hamster neu proto-oncogene have been observed in the transmembrane domain of N-nitroso-N-ethylurea (ENU)-induced neurofibromas. Genomic DNA derived from tumor tissue showed transforming activity in an NIH 3T3 assay and a cDNA clone of the hamster neu gene, containing the entire protein-coding region, was isolated from a transformant cDNA library. The encoded product is 92 and 88% homologous to the rat neu and the human c-erbB-2, respectively. The product of the mutated hamster neu gene showed increased autophosphorylation of Tyr residues.

• Authors: T Nakamura, T Ushijima, Y Ishizaka, M Nagao, M Arai, Y Yamazaki, T Ishikawa
• Journal: Gene (Gene) Vol. 140 Issue 2 Pg. 251-5 (Mar 25 1994) ISSN: 0378-1119 [Print] Netherlands
• PMID: 7908275 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MAS1 protein, human
• Proto-Oncogene Mas
• Proto-Oncogene Proteins
• Tyrosine
• ErbB Receptors
• Receptor, ErbB-2

Topics

• 3T3 Cells
• Amino Acid Sequence
• Animals
• Base Sequence
• Cloning, Molecular
• Cricetinae
• ErbB Receptors (genetics)
• Gene Expression Regulation
• Humans
• Mesocricetus
• Mice
• Molecular Sequence Data
• Phosphorylation
• Proto-Oncogene Mas
• Proto-Oncogene Proteins (genetics)
• Proto-Oncogenes
• Rats
• Receptor, ErbB-2
• Sequence Homology, Amino Acid
• Sequence Homology, Nucleic Acid
• Transfection
• Transformation, Genetic
• Tyrosine (metabolism)