Analysis of effector T cells against the murine syngeneic tumor MethA in mice orally administered antitumor polysaccharide SPR-901.

Abstract

The growth of MethA tumor was significantly inhibited by oral administration of the alpha-glucan SPR-901 in BALB/c (+/+) mice but not in nude mice. Mice treated orally with SPR-901 exhibited an augmentation of antigen-specific resistance against rechallenge with the tumor cells. The tumor-neutralizing activity of regional lymph node cells from MethA-bearing mice against the tumor was augmented by oral administration of SPR-901. The tumor-neutralizing activity of lymph node cells from SPR-901-treated mice mainly appeared in Lyt2+ cells. Furthermore, lymphokine-activated killer activity of these cells was enhanced by administration of SPR-901. The antitumor effect of SPR-901 was abrogated in mice depleted of either L3T4+ or Lyt2+ cells, and in cyclosporin-A-treated mice. These results suggest that Lyt2+ cells are important effector cells in MethA-bearing mice orally administered SPR-901 and that functional exertion of both Lyt2+ and L3T4+ T cells is necessary for the antitumor effect of orally administered SPR-901 in vivo.

• Authors: Y Takeda, M Tanaka, H Miyazaki, S Takeo, K Nomoto, Y Yoshikai
• Journal: Cancer immunology, immunotherapy : CII (Cancer Immunol Immunother) Vol. 38 Issue 3 Pg. 143-8 (Mar 1994) ISSN: 0340-7004 [Print] Germany
• PMID: 7907272 (Publication Type: Journal Article)

Chemical References

• Adjuvants, Immunologic
• Antigens, Ly
• Antineoplastic Agents
• Glucans
• rice bran saccharide
• Cyclosporine

Topics

• Adjuvants, Immunologic
• Administration, Oral
• Animals
• Antigens, Ly (immunology)
• Antineoplastic Agents
• CD4-Positive T-Lymphocytes (immunology)
• Cyclosporine (pharmacology)
• Female
• Glucans (administration & dosage)
• Immunotherapy
• Killer Cells, Lymphokine-Activated (immunology)
• Lymph Nodes (cytology)
• Lymphocyte Depletion
• Mice
• Mice, Inbred BALB C
• Sarcoma, Experimental (therapy)
• T-Lymphocyte Subsets (immunology)