Delayed-type hypersensitivity response to human papillomavirus type 16 E6 protein in a mouse model.

Abstract

A mouse model incorporating the epitheliotropic nature of human papillomavirus (HPV) infections has been used to study an immune response to HPV type 16 (HPV-16) E6 protein in vivo. Using a transplantation technique, a novel immortal keratinocyte cell line expressing the E6 protein has been grafted onto syngeneic mice to re-form a differentiated epithelium overlying a granulation tissue bed. By this approach the presentation of viral antigens to the immune system can be modelled in a way analogous to the natural infection. Here we report a delayed-type hypersensitivity (DTH) reaction in grafted mice challenged intradermally with a recombinant vaccinia virus expressing the HPV-16 E6 protein. The specificity of the response was confirmed by the absence of a DTH reaction to challenge with virus expressing either HPV-16 E7 or L1 protein.

• Authors: M A Chambers, S N Stacey, J R Arrand, M A Stanley
• Journal: The Journal of general virology (J Gen Virol) Vol. 75 ( Pt 1) Pg. 165-9 (Jan 1994) ISSN: 0022-1317 [Print] England
• PMID: 7906707 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral
• E6 protein, Human papillomavirus type 16
• Oncogene Proteins, Viral
• Recombinant Proteins
• Repressor Proteins

Topics

• Animals
• Antigens, Viral (biosynthesis, immunology)
• CD4-Positive T-Lymphocytes (immunology)
• Cell Line
• Hypersensitivity, Delayed (immunology)
• Keratinocytes (transplantation, virology)
• Mice
• Mice, Inbred BALB C
• Oncogene Proteins, Viral (biosynthesis, immunology)
• Papillomaviridae (immunology)
• Recombinant Proteins (biosynthesis, immunology)
• Repressor Proteins
• Sensitivity and Specificity
• Vaccinia virus (genetics)