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Use of proteinase 3 purified by reverse phase HPLC to detect autoantibodies in systemic vasculitis.

Abstract
The vasculitis autoantigen proteinase 3 was purified from neutrophil primary granules using reverse phase high performance liquid chromatography. It was shown to be free of important contaminants, was enzymatically active and was antigenic to sera containing antineutrophil cytoplasmic antibodies with a cytoplasmic pattern (C-ANCA) by indirect immunofluorescence. Development of an enzyme-linked immunosorbent assay showed that this preparation could be used to detect autoantibodies in Wegener's granulomatosis and microscopic polyangiitis. Assays based on reverse phase HPLC-purified proteinase 3 will be valuable in diagnosis and monitoring of treatment, and should increase our understanding of the autoimmune response in primary systemic vasculitis.
AuthorsG Gaskin, H Kendal, A Coulthart, N Turner, C D Pusey
JournalJournal of immunological methods (J Immunol Methods) Vol. 180 Issue 1 Pg. 25-33 (Mar 13 1995) ISSN: 0022-1759 [Print] Netherlands
PMID7897245 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Antineutrophil Cytoplasmic
  • Antibodies, Monoclonal
  • Autoantibodies
  • Autoantigens
  • Serine Endopeptidases
  • Myeloblastin
Topics
  • Amino Acid Sequence
  • Antibodies, Antineutrophil Cytoplasmic
  • Antibodies, Monoclonal
  • Autoantibodies (analysis, immunology)
  • Autoantigens (immunology, isolation & purification)
  • Chromatography, High Pressure Liquid (methods)
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay (methods)
  • Granulomatosis with Polyangiitis (immunology)
  • Humans
  • Molecular Sequence Data
  • Myeloblastin
  • Neutrophils (enzymology)
  • Serine Endopeptidases (immunology, isolation & purification)
  • Vasculitis (immunology)

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