Bauhinia purpurea lectin (BPA) binding of rat type I pneumocytes: alveolar epithelial alterations after radiation-induced lung injury.

Abstract

In the rat lung, we found that the Bauhinia purpurea lectin (BPA) specifically binds to the type I alveolar epithelial cells and to the alveolar macrophages. Double label fluorescence employing FITC-coupled Maclura pomifera lectin (MPA) and bBPA-avidin-Texas Red showed that BPA binding was confined to type I cells. In addition, a minor staining of the luminal border of the terminal bronchiolar epithelium was found. The sequence of tissue injury following X-radiation was examined in rats. BPA is a suitable marker which indicates epithelial changes during the early stage of pneumonitis and the subsequent development of fibrosis.

• Authors: M Kasper, D Schuh, M Müller
• Journal: Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie (Exp Toxicol Pathol) Vol. 46 Issue 4-5 Pg. 361-7 (Oct 1994) ISSN: 0940-2993 [Print] Germany
• PMID: 7894248 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bauhinia purpurea lectin
• Lectins
• Plant Lectins
• maclurin

Topics

• Animals
• Disease Models, Animal
• Dose-Response Relationship, Radiation
• Epithelium (metabolism, pathology, radiation effects)
• Histocytochemistry
• Immunohistochemistry
• Lectins (metabolism)
• Lung (pathology, radiation effects)
• Macrophages, Alveolar (metabolism, pathology, radiation effects)
• Plant Lectins
• Pulmonary Alveoli (metabolism, pathology, radiation effects)
• Pulmonary Fibrosis (metabolism, pathology)
• Radiation Injuries, Experimental (metabolism, pathology)
• Radiation Pneumonitis (metabolism, pathology)
• Rats
• Rats, Inbred F344