Antitumor activity of a spicamycin derivative, KRN5500, and its active metabolite in tumor cells.

Abstract

KRN5500, (6-[4-Deoxy-4-(2E,4E)-tetradecadienoylglycyl]amino-L-glycero - beta-L-mannoheptopyranosyl]amino-9H-purine), was semi-synthesized in an attempt to increase the therapeutic effects of spicamycin analogues. The present study evaluated the antitumor activity of KRN5500 against murine tumors and human tumor xenografts. KRN5500 prolonged the survival of P388 leukemia- and B16 melanoma-bearing mice but was marginally effective on colon adenocarcinoma 26. The antitumor activity of KRN5500 (4 mg/kg/day x 5, IV) against xenografts of 10 human stomach, 14 colon and 2 esophageal cancers was evaluated with two parameters: the tumor growth inhibition rate (TGIR) and the tumor mass reduction by comparison with mitomycin C (MMC, 6.7 mg/kg/day x 1,IV). KRN5500 showed a marked efficacy in the human tumor xenograft model. The overall response rate of 26 cancers to KRN5500, evaluated by TGIR, was approximately equal to their response rate to MMC (72% vs. 73%). However, more tumors were reduced by KRN5500 than by MMC (52% vs. 39%). It is notable that the response rates of 14 colon cancers to KRN5500 were significantly higher than those to MMC, both in TGIR (69% vs. 58%) and in tumor mass reduction (46% vs. 23%). Among the tumors sensitive to KRN5500, COL-1 showed a marked response (TGIR 93%) and a significant reduction in tumor mass (0.22-fold the starting volume). In the mode of action, KRN5500 was found to show an inhibitory effect on protein synthesis in P388 cells (IC50 1.5 microM). However, KRN5500 was ineffective even at 170 microM in inhibition of protein synthesis in rabbit reticulocyte lysates.(ABSTRACT TRUNCATED AT 250 WORDS)

• Authors: M Kamishohara, H Kawai, T Sakai, T Isoe, K Hasegawa, J Mochizuki, T Uchida, S Kataoka, H Yamaki, T Tsuruo
• Journal: Oncology research (Oncol Res) Vol. 6 Issue 8 Pg. 383-90 ( 1994) ISSN: 0965-0407 [Print] United States
• PMID: 7894087 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Protein Synthesis Inhibitors
• Purine Nucleosides
• Mitomycin
• KRN 5500

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Female
• Humans
• Mice
• Mice, Inbred BALB C
• Mice, Inbred DBA
• Mice, Inbred Strains
• Mice, Nude
• Mitomycin (pharmacology)
• Neoplasms, Experimental (drug therapy)
• Protein Synthesis Inhibitors (pharmacology)
• Purine Nucleosides (antagonists & inhibitors, metabolism, pharmacology)
• Subrenal Capsule Assay