Dysregulation of interleukin 8, interleukin 10, and interleukin 12 release by alveolar macrophages from HIV type 1-infected subjects.

Abstract

We examined the in vitro release of interleukin 8 (IL-8), interleukin 10 (IL-10), and interleukin 12 (IL-12) by alveolar macrophages from normal volunteers and HIV-1-infected subjects. Normal volunteers had very low levels of IL-8 and IL-10 and undetectable IL-12 in the cell-free bronchoalveolar lavage fluid (BALF). Asymptomatic HIV-1-infected subjects had elevated levels of IL-8 and IL-10 in their BALF, and HIV-1-infected subjects with nonspecific interstitial pneumonitis (NIP) or infected with Pneumocystis carinii had the highest BALF levels of IL-10 and IL-8. It was found that alveolar macrophages from asymptomatic HIV-1 subjects and from NIP subjects spontaneously released elevated IL-8, IL-10, and IL-12. However, AIDS subjects infected with P. carinii had cells that released elevated levels of IL-10 and IL-8, but low levels of IL-12. When alveolar macrophages were stimulated with Staphylococcus aureus Cowan (SAC), cells from normal volunteers responded with a considerably increased release of IL-8, IL-10, and IL-12; cells from HIV-1-infected subjects without P. carinii infection responded with a moderate increase in release of all three monokines. SAC stimulation did not enhance the release of monokines by cells from AIDS subjects with P. carinii infection, and IL-12 levels remained low. There was no strict relationship between spontaneous cytokine release and p24 HIV-1 antigen expression by alveolar macrophages. Finally, we showed that neutralizing IL-10 production by alveolar macrophages from AIDS subjects substantially increased IL-12 releasability.(ABSTRACT TRUNCATED AT 250 WORDS)

• Authors: M Denis, E Ghadirian
• Journal: AIDS research and human retroviruses (AIDS Res Hum Retroviruses) Vol. 10 Issue 12 Pg. 1619-27 (Dec 1994) ISSN: 0889-2229 [Print] United States
• PMID: 7888221 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HIV Core Protein p24
• Interleukin-8
• Lipopolysaccharides
• Interleukin-10
• Interleukin-12

Topics

• Acquired Immunodeficiency Syndrome (complications, metabolism)
• Bronchoalveolar Lavage Fluid (virology)
• Genes, gag
• HIV Core Protein p24 (metabolism)
• HIV-1 (immunology)
• Humans
• Interleukin-10 (biosynthesis, immunology, metabolism)
• Interleukin-12 (metabolism)
• Interleukin-8 (biosynthesis, metabolism)
• Lipopolysaccharides (pharmacology)
• Lung Diseases, Interstitial (metabolism)
• Macrophages, Alveolar (metabolism, pathology, virology)
• Staphylococcus aureus (physiology)