HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Application of a monoclonal antibody to estimate rabbit fibrinopeptide A released by habutobin.

Abstract
We reported previously that habutobin, one of the type A thrombin-like enzymes, releases fibrinopeptide A alone from rabbit fibrinogen. To evaluate the effective action of habutobin in experiments using rabbit for the treatment of thrombosis, we attempted to develop an immunological method for measuring the fibrinopeptide A level in the circulating blood of rabbit. The purified rabbit fibrinopeptide A was coupled to keyhole limpet hemocyanin and BALB/c mice were immunized with the resultant fibrinopeptide A-hemocyanin conjugate. The spleen cells of an immunized mouse were fused with myeloma cells (P3-X63-Ag8-U1). As a result, one hybridoma (a-F-7) was selected, which secreted an antibody against rabbit fibrinopeptide A. Using this monoclonal antibody, we developed a competitive enzyme-linked immunoassay for estimating rabbit fibrinopeptide A. It was able to measure rabbit fibrinopeptide A contained in bentonite defibrinated plasma. This competitive enzyme-linked immunoassay should be useful for determining the fibrinopeptide A level in the circulating blood of rabbits, using plasma defibrinated by bentonite.
AuthorsK Kinjoh, M Nakamura, T Kosugi
JournalToxicon : official journal of the International Society on Toxinology (Toxicon) Vol. 32 Issue 11 Pg. 1413-23 (Nov 1994) ISSN: 0041-0101 [Print] England
PMID7886699 (Publication Type: Journal Article)
Chemical References
  • Adjuvants, Immunologic
  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • Blood Proteins
  • Crotalid Venoms
  • Fibrinopeptide A
  • Fibrinopeptide B
  • Hemocyanins
  • Serine Endopeptidases
  • habutobin
  • keyhole-limpet hemocyanin
Topics
  • Adjuvants, Immunologic (metabolism)
  • Animals
  • Antibodies, Monoclonal (chemistry, immunology, isolation & purification)
  • Antibody Specificity
  • Antigen-Antibody Complex
  • Binding, Competitive
  • Blood Proteins (metabolism)
  • Cell Fusion
  • Chromatography, Ion Exchange
  • Crotalid Venoms (metabolism)
  • Enzyme-Linked Immunosorbent Assay
  • Fibrinopeptide A (analysis, immunology, isolation & purification, metabolism)
  • Fibrinopeptide B (analysis, immunology, isolation & purification)
  • Hemocyanins (metabolism)
  • Hybridomas
  • Hydrogen-Ion Concentration
  • Mice
  • Mice, Inbred BALB C
  • Rabbits
  • Serine Endopeptidases (metabolism)
  • Spleen (cytology)
  • Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: