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Sequence, expression and transcriptional analysis of the coronafacate ligase-encoding gene required for coronatine biosynthesis by Pseudomonas syringae.

Abstract
Pseudomonas syringae pv. glycinea PG4180 produces the chlorosis-inducing phytotoxin coronatine (COR), which consists of a polyketide component, coronafacic acid (CFA), ligated by an amide bond to coronamic acid (CMA), an ethylcyclopropyl amino-acid derived from isoleucine. We report the nucleotide sequence of a 2.37-kb region containing the coronafacate ligase-encoding gene (cfl) which is required for the amide linkage of CFA and CMA. The transcription start point for cfl was identified, and the Cfl protein was overproduced from the T7lac promoter in Escherichia coli. The deduced amino-acid sequence of Cfl showed homology to a variety of adenylate-forming enzymes which bind and hydrolyze ATP in order to activate their substrates for further ligation.
AuthorsH Liyanage, C Penfold, J Turner, C L Bender
JournalGene (Gene) Vol. 153 Issue 1 Pg. 17-23 (Feb 3 1995) ISSN: 0378-1119 [Print] NETHERLANDS
PMID7883180 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Amino Acids
  • Bacterial Proteins
  • Enzymes
  • Indenes
  • coronatine
  • Ligases
  • Amide Synthases
  • Cfl protein, Pseudomonas syringae
Topics
  • Amide Synthases
  • Amino Acid Sequence
  • Amino Acids (metabolism)
  • Bacterial Proteins
  • Base Sequence
  • Enzymes (classification)
  • Escherichia coli (genetics)
  • Genes, Bacterial
  • Indenes (metabolism)
  • Ligases (genetics)
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Pseudomonas (genetics, metabolism)
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcription, Genetic

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