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Exogenous catalase introduced in CHO cells by electroporation does not protect against chromosome damage induced by ionizing radiation.

Abstract
The relative importance of hydrogen peroxide generated as a consequence of irradiation with X-rays for the production of chromosomal aberrations has been studied in cultured CHO cells. Catalase introduced into cells by electroporation protected DNA from strand breakage induced by hydrogen peroxide given 4h later, and the yield of chromosome aberrations was also reduced. Nevertheless, when the cells were irradiated after treatment with catalase following a similar protocol and the yield of chromosomal aberrations analyzed at metaphase, no protective effect was observed as compared with cells treated with X-rays alone. These observations seem to support the hypothesis that hydroxyl radicals generated from hydrogen peroxide are not a major factor responsible for chromosome damage induced by ionizing radiation.
AuthorsF Cortés, I Domínguez, B Sandström
JournalCell biology international (Cell Biol Int) Vol. 18 Issue 10 Pg. 959-65 (Oct 1994) ISSN: 1065-6995 [Print] England
PMID7881383 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Hydroxyl Radical
  • DNA
  • Catalase
Topics
  • Animals
  • CHO Cells
  • Catalase (administration & dosage, metabolism, pharmacology)
  • Chromosome Aberrations
  • Cricetinae
  • DNA (drug effects, radiation effects)
  • DNA Damage
  • Electroporation
  • Hydroxyl Radical (metabolism)

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