Effect of proton pump inhibitor on cell growth and sensitivity to cis-diamminedichloroplatinum(II) in non-small cell lung cancer cell lines.

Abstract

Recently, the importance of the potassium pump in the cellular accumulation of cis-diamminedichloroplatinum(II) (CDDP) has been reported. In this study we evaluated the role of the proton pump as a determinant of the sensitivity to CDDP in non-small cell lung cancer cell lines in vitro by using a selective proton pump inhibitor, AG-2000. PC-9 and PC-9/CDDP cell lines, which are sensitive or resistant to CDDP, were used for these experiments. PC-9/CDDP was 17.4-fold more resistant to CDDP than PC-9 cells. Relative resistance was not altered by co-incubation with a non-cytotoxic dosage of AG-2000. From these studies, it was shown that the proton pump inhibitor AG-2000 did not enhance the sensitivity to CDDP. However, as AG-2000 is not cytotoxic and does not compromise the CDDP-sensitivity in NSCLC cells at the concentration of clinical use for gastroduodenal ulcer, AG-2000 can be used with CDDP in chemotherapy for lung cancer.

• Authors: T Bando, K Kasahara, K Shibata, Y Nakatsumi, M Fujimura, T Matsuda
• Journal: Anticancer research (Anticancer Res) 1994 Nov-Dec Vol. 14 Issue 6B Pg. 2727-30 ISSN: 0250-7005 [Print] Greece
• PMID: 7872708 (Publication Type: Journal Article)

Chemical References

• Benzimidazoles
• Proton Pump Inhibitors
• Pyridines
• AG 2000
• Cisplatin

Topics

• Adenocarcinoma
• Benzimidazoles (pharmacology)
• Carcinoma, Non-Small-Cell Lung
• Cell Division (drug effects)
• Cell Line
• Cisplatin (toxicity)
• Clone Cells
• Dose-Response Relationship, Drug
• Drug Resistance
• Humans
• Kinetics
• Lung Neoplasms
• Proton Pump Inhibitors
• Pyridines (pharmacology)
• Sensitivity and Specificity
• Tumor Cells, Cultured