The effect of intravenous and intra-tumoural chemotherapy using a monoclonal antibody-drug conjugate in a xenograft model of pancreatic cancer.

Abstract

In order to investigate the efficacy of the intra-tumoural administration of an anticancer drug-monoclonal antibody conjugate in athymic nude mice bearing xenografts of a human pancreatic carcinoma, we examined the clearance of the murine monoclonal antibody A7 from the xenografts after intravenous or intra-tumoural administration and measured the antitumour effect of neocarzinostatin conjugated to MAb A7 following intravenous or intra-tumoural injection. Compared with 125I-labelled normal mouse IgG, a larger amount of 125I-labelled A7 remained in the tumour after both intravenous and intra-tumoural injection, and a significantly larger amount of 125I-labelled A7 remained in the tumour after intra-tumoural injection than that after intravenous injection. Moreover, a larger amount of 125I-labelled A7-NCS localized in the tumour after intra-tumoural injection than that after intravenous injection. Neocarzinostatin conjugated to MAb A7 showed greater activity against human pancreatic cancer than neocarzinostatin alone after both intravenous and intra-tumoural administration. Tumour growth was suppressed completely by the intra-tumoural administration of A7-NCS at a dose that did not suppress tumour growth via the intravenous route. These observations suggest that the intra-tumoural injection of neocarzinostatin conjugated to MAb A7 offers promise in treating pancreatic carcinoma.

• Authors: E Otsuji, T Yamaguchi, H Tsuruta, Y Yata, H Nishi, K Okamoto, K Taniguchi, M Kato, T Kotani, K Kitamura
• Journal: European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology (Eur J Surg Oncol) Vol. 21 Issue 1 Pg. 61-5 (Feb 1995) ISSN: 0748-7983 [Print] England
• PMID: 7851556 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Immunotoxins
• Iodine Radioisotopes
• Zinostatin

Topics

• Adenocarcinoma (drug therapy)
• Animals
• Antibodies, Monoclonal (therapeutic use)
• Humans
• Immunotoxins (administration & dosage, therapeutic use)
• Infusions, Intravenous
• Injections, Intralesional
• Iodine Radioisotopes
• Mice
• Mice, Nude
• Pancreatic Neoplasms (drug therapy)
• Transplantation, Heterologous
• Tumor Cells, Cultured
• Zinostatin (administration & dosage, therapeutic use)