Sulofenur cytotoxicity and changes in cytosolic calcium and mitochondrial membrane potential in human colon adenocarcinoma cell lines.

Abstract

Sulofenur treatment (12.5 microM-1 mM) of colon adenocarcinoma cell lines resulted in dose- and time-dependent cell killing. LYc5 cells were viable longer than GC3/c1 cells. Each concentration resulted in elevation of cytosolic calcium [Ca2+]i) for both cell lines. At lower doses, elevation was delayed for LYc5 cells. GC3/c1 cells after 1 mM treatment in Ca(2+)-free HBSS showed no rise of [Ca2+]i. GC3/c1 cells after carbonyl cyanide-m-chlorophenylhydrazone rapidly lost rhodamine 123 fluorescence from mitochondria; after 1 mM sulofenur, fluorescence faded slowly. Following treatment, cells became rounded, blebs formed and the cells died. Results suggest that elevated [Ca2+]i plays an important role in sulofenur cytotoxicity.

• Authors: P C Phelps, P T Jain, I K Berezesky, G B Boder, B F Trump
• Journal: Cancer letters (Cancer Lett) Vol. 88 Issue 1 Pg. 27-35 (Jan 06 1995) ISSN: 0304-3835 [Print] Ireland
• PMID: 7850770 (Publication Type: Journal Article)

Chemical References

• Sulfonylurea Compounds
• Calcium
• sulofenur

Topics

• Adenocarcinoma (pathology)
• Calcium (metabolism)
• Cell Death (drug effects)
• Cell Survival (drug effects)
• Colonic Neoplasms (pathology)
• Cytosol (metabolism)
• Dose-Response Relationship, Drug
• Humans
• In Vitro Techniques
• Membrane Potentials (drug effects)
• Microscopy, Electron
• Mitochondria (physiology)
• Sulfonylurea Compounds (toxicity)
• Tumor Cells, Cultured