Hybridocytochemical and immuno-ultrastructural study of calcitonin gene expression in cultured medullary carcinoma cells.

Abstract

The study was aimed at a morphological demonstration of calcitonin (CT) gene expression in cultured TT cells, or, more specifically, hybridocytochemical detection of CT mRNA and calcitonin gene-related peptide (CGRP) mRNA and ultrastructural localization of the two hormones. The TT cells originated from medullary carcinoma of human thyroid gland. Ultrastructural studies of TT cells demonstrated a well-developed rough endoplasmic reticulum, large Golgi apparatus and low number of secretory granules. Hybridocytochemical studies showed the presence of mRNAs for CT and CGRP in all TT cells. At the ultrastructural level, double immunolabelling demonstrated that the two hormones were always expressed together in the same secretory granules. Our results provide a significant addition to the biochemical studies performed up to now and indicate that all TT cells produce both mRNAs and both hormones in parallel.

• Authors: M Zabel, J Seidel, A Kaczmarek, J Surdyk-Zasada, J Grzeszkowiak, A Górny
• Journal: Histochemistry (Histochemistry) Vol. 102 Issue 4 Pg. 323-7 (Oct 1994) ISSN: 0301-5564 [Print] Germany
• PMID: 7843995 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Messenger
• Calcitonin
• Calcitonin Gene-Related Peptide

Topics

• Calcitonin (analysis)
• Calcitonin Gene-Related Peptide (analysis)
• Carcinoma, Medullary (chemistry, ultrastructure)
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry
• Microscopy, Electron
• RNA, Messenger (analysis)
• Thyroid Neoplasms (chemistry, ultrastructure)
• Tumor Cells, Cultured