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Structure and function of a long alternating purine-pyrimidine sequence in the mouse alcohol dehydrogenase Adh-1 gene.

Abstract
The mouse alcohol dehydrogenase gene Adh-1 contains an unusually long alternating purine-pyrimidine sequence within its first intron. This alternating sequence differs in length between strains that differ in the extent of Adh-1 expression, and it has been suggested that it plays a role in gene expression. We demonstrate that this alternating sequence can form Z-DNA in vitro. The alternating sequence can act as a positive regulatory element in transient transfection assays in hepatoma cell lines, but not in CV-1 (monkey kidney) cells, suggesting that it can act as a tissue-specific regulatory element. Nuclear run-on experiments showed that the differential expression of Adh-1 from high- and low-activity strains is, however, controlled at the post-transcription level.
AuthorsM Yu, H J Edenberg
JournalArchives of biochemistry and biophysics (Arch Biochem Biophys) Vol. 316 Issue 1 Pg. 407-12 (Jan 10 1995) ISSN: 0003-9861 [Print] United States
PMID7840644 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Purines
  • Pyrimidines
  • Recombinant Proteins
  • DNA
  • Alcohol Dehydrogenase
Topics
  • Alcohol Dehydrogenase (biosynthesis, genetics)
  • Animals
  • Cell Nucleus (metabolism)
  • DNA (genetics)
  • Gene Expression Regulation, Enzymologic (genetics)
  • Introns (genetics)
  • Liver (metabolism)
  • Mice
  • Purines
  • Pyrimidines
  • RNA Processing, Post-Transcriptional
  • Recombinant Proteins (biosynthesis)
  • Transcription, Genetic
  • Tumor Cells, Cultured

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