Abstract |
The mouse alcohol dehydrogenase gene Adh-1 contains an unusually long alternating purine- pyrimidine sequence within its first intron. This alternating sequence differs in length between strains that differ in the extent of Adh-1 expression, and it has been suggested that it plays a role in gene expression. We demonstrate that this alternating sequence can form Z-DNA in vitro. The alternating sequence can act as a positive regulatory element in transient transfection assays in hepatoma cell lines, but not in CV-1 (monkey kidney) cells, suggesting that it can act as a tissue-specific regulatory element. Nuclear run-on experiments showed that the differential expression of Adh-1 from high- and low-activity strains is, however, controlled at the post-transcription level.
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Authors | M Yu, H J Edenberg |
Journal | Archives of biochemistry and biophysics
(Arch Biochem Biophys)
Vol. 316
Issue 1
Pg. 407-12
(Jan 10 1995)
ISSN: 0003-9861 [Print] United States |
PMID | 7840644
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Purines
- Pyrimidines
- Recombinant Proteins
- DNA
- Alcohol Dehydrogenase
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Topics |
- Alcohol Dehydrogenase
(biosynthesis, genetics)
- Animals
- Cell Nucleus
(metabolism)
- DNA
(genetics)
- Gene Expression Regulation, Enzymologic
(genetics)
- Introns
(genetics)
- Liver
(metabolism)
- Mice
- Purines
- Pyrimidines
- RNA Processing, Post-Transcriptional
- Recombinant Proteins
(biosynthesis)
- Transcription, Genetic
- Tumor Cells, Cultured
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