HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Endothelin-1 induced middle cerebral artery occlusion: pathological consequences and neuroprotective effects of MK801.

Abstract
In the present study we utilise the potent vasoconstrictor properties of endothelin-1 (Et-1) in a new model of middle cerebral artery occlusion in the anaesthetized rat. We evaluate the reproducibility of the model and examine the neuroprotective efficacy of the potent anti-ischaemic agent, MK801. Adult male SD rats received MK801 (5 mg/kg, n = 7) or saline vehicle (n = 7) 30 mins prior to the microinjection of Et-1 (60 pmol in 3 microliters) via a 31-g cannula stereotaxically positioned 0.5 mm above the middle cerebral artery. Three days after the injection of Et-1, rats were perfusion fixed, the brain removed, cryostat sectioned and processed for histological staining. Sections at eight predetermined levels were examined by light microscopy and the volume of infarction calculated. Following administration of Et-1, saline-pretreated rats exhibited a pattern of ischaemic damage similar to that previously reported following permanent occlusion of the rat middle cerebral artery. This pattern was characterised by a large volume of infarction covering the dorsal and lateral neocortex (98 +/- 12 mm3) and striatum (32 +/- 3 mm3) ipsilateral to the insult. Power analysis predicted a group size of 7 would be required for a 50% reduction in ischaemic damage to be recorded as statistically significant at the 5% level. Pretreatment with MK801 reduced cortical tissue damage by 51% (P = 0.026) but did not significantly alter either the pattern or volume of infarction (33 +/- 4 mm3; P = 0.95) in the striatum.(ABSTRACT TRUNCATED AT 250 WORDS)
AuthorsJ Sharkey, S P Butcher, J S Kelly
JournalJournal of the autonomic nervous system (J Auton Nerv Syst) Vol. 49 Suppl Pg. S177-85 (Sep 1994) ISSN: 0165-1838 [Print] Netherlands
PMID7836677 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Anesthetics
  • Endothelins
  • Receptors, Endothelin
  • Dizocilpine Maleate
Topics
  • Anesthetics (pharmacology)
  • Animals
  • Brain (pathology)
  • Brain Ischemia (chemically induced, pathology)
  • Cerebral Arteries (drug effects)
  • Dizocilpine Maleate (pharmacology)
  • Endothelins (administration & dosage, metabolism, pharmacology)
  • Injections
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Endothelin (drug effects, metabolism)
  • Stereotaxic Techniques
  • Vasoconstriction (drug effects)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: