Human high molecular weight-melanoma associated antigen mimicry by mouse anti-idiotypic MAb MK2-23. Immunohistochemical analysis of the reactivity of anti-anti-idiotypic MAb with surgically removed melanoma lesions.

Abstract

The mouse anti-id MAb MK2-23 bears the internal image of the antigenic determinant defined by anti-HMW-MAA MAb 763.74. Active specific immunotherapy with anti-id MAb MK2-23 is associated with a statistically significant prolongation of survival in patients with malignant melanoma. Characterization of the immune response elicited by anti-id MAb MK2-23 may contribute to our understanding of the mechanisms underlying the apparent beneficial clinical effects of active specific immunotherapy with anti-id MAb MK2-23. Therefore, 8 HMW-MAA binding anti-anti-id MAbs elicited with MAb MK2-23 were characterized in their reactivity with a large panel of surgically removed benign and malignant tumors and of normal tissues. The 8 anti-anti-id MAbs displayed subtle differences in their immunoperoxidase staining of both benign and malignant lesions and of normal tissues. The diversity in the fine specificity of the 8 anti-anti-id MAbs is likely to reflect the few somatic mutations which occur in the amino-acid sequence of the variable regions of their heavy and light chains in the course of the immune response to MAb MK2-23. The reactivity patterns of the 8 anti-anti-id MAbs with the tissue substrates are similar, although not superimposable upon that of anti-HMW-MAA MAb 763.74 elicited with melanoma cells. This difference may reflect the imperfect mimicry by anti-id MAb MK2-23 of the antigenic determinant defined by anti-HMW-MAA MAb 763.74.

• Authors: T Kageshita, S Hirai, T Ono, S Ferrone
• Journal: International journal of cancer (Int J Cancer) Vol. 60 Issue 3 Pg. 334-40 (Jan 27 1995) ISSN: 0020-7136 [Print] United States
• PMID: 7829240 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Anti-Idiotypic
• Antibodies, Neoplasm
• Antigens, Neoplasm
• Melanoma-Specific Antigens
• Neoplasm Proteins

Topics

• Animals
• Antibodies, Anti-Idiotypic (immunology)
• Antibodies, Neoplasm (immunology)
• Antibody Specificity
• Antigens, Neoplasm (immunology)
• Humans
• Immunoenzyme Techniques
• Melanoma (immunology)
• Melanoma-Specific Antigens
• Mice
• Neoplasm Proteins (immunology)
• Nevus (immunology)
• Skin Neoplasms (immunology)