Abstract |
The effect of the nucleotides and a nucleotide mixture (OG-VI), consisting of inosine, guanosine 5'-monophosphate (5'-GMP), cytidine, uridine, thymidine (TdR) (4:4:4:3:1 in molar ratio), and TdR co-administration on proliferation of KATO III human gastric cancer cells in culture was evaluated. Consumption of purine and pyrimidine by cancer cells and changes in cell number with OG-VI or TdR were compared with the control culture medium (Williams E) after 72 hour-culture. Addition of OG-VI or TdR did not enhance the cellular proliferation, but inhibited growth when given in higher concentrations (0.3-3 mM inosine, 0.3-3 mM 5'-GMP, 0.22-2.2 mM uridine, 74-740 microM TdR). Consumption rate of TdR in the medium was less in the TdR group, 33.7%, than in the OG-VI group, 72.2% (p < 0.05). This suggests that TdR metabolism is modulated by other nucleosides and nucleotide included in OG-VI. Under the coadministration of 5-fluorouracil (FUra), addition of OG-VI or TdR suppressed cellular proliferation (p < 0.05). The inhibition rate of cellular proliferation in the OG-VI group was slightly higher than the TdR group, but there was no statistically significant difference between the two groups. The combination of FUra with OG-VI or TdR enhances the antitumor effect of FUra. It is concluded that the OG-VI does not enhance the tumor cell proliferation and it is a potential biochemical modulator of FUra metabolism in human cancer cells.
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Authors | J Wang, M Usami, I Yasuda, H Kasahara, G Kotani, Y Cao, J Zheng, A Iso, T Kanamaru, H Ohyanagi |
Journal | The Kobe journal of medical sciences
(Kobe J Med Sci)
Vol. 40
Issue 2
Pg. 65-75
(Apr 1994)
ISSN: 0023-2513 [Print] Japan |
PMID | 7823535
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Drug Combinations
- Nucleosides
- Nucleotides
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Topics |
- Cell Division
(drug effects)
- Drug Combinations
- Humans
- Nucleosides
(pharmacology)
- Nucleotides
(pharmacology)
- Stomach Neoplasms
(pathology)
- Tumor Cells, Cultured
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