Cloning of the Dck gene encoding rat deoxycytidine kinase.

Abstract

In order to study the mutational inactivation of deoxycytidine kinase (Dck) in a rat model for acute myeloid leukemia we have cloned the complete coding region of the rat Dck gene. Using primers chosen from the human Dck cDNA sequence, we obtained a rat-specific probe via PCR and used it to isolate two clones from a rat lymphocyte cDNA library. The ORF showed 89.7 and 92.2% nucleotide identity with the human and mouse Dck, respectively, and encodes a 260-amino-acid protein, that is 91.9 and 94.6% homologous to human and mouse Dck, respectively. Northern blot analysis of rat tissues revealed high expression of a 4.1-kb Dck transcript in the thymus, whereas spleen, liver and lung samples showed weak expression of the gene. This tissue-specific expression pattern was confirmed by cDNA-PCR analysis.

• Authors: A P Stegmann, M W Honders, R Willemze, J E Landegent
• Journal: Gene (Gene) Vol. 150 Issue 2 Pg. 351-4 (Dec 15 1994) ISSN: 0378-1119 [Print] Netherlands
• PMID: 7821805 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• DNA, Complementary
• Recombinant Proteins
• Deoxycytidine Kinase

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Cloning, Molecular
• Conserved Sequence
• DNA Primers
• DNA, Complementary
• Deoxycytidine Kinase (antagonists & inhibitors, biosynthesis, genetics)
• Humans
• Mice
• Molecular Sequence Data
• Open Reading Frames
• Organ Specificity
• Polymerase Chain Reaction
• Rats (genetics)
• Recombinant Proteins (antagonists & inhibitors, biosynthesis)
• Sequence Homology, Amino Acid