Abstract |
1,1'-Bis(4-anilino-5-naphthalenesulphonic acid) ( bis-ANS) has been shown by fluorescence spectroscopy to bind to bromelain-cleaved influenza haemagglutinin ( BHA). The fluorescence intensity of 1.2 microM bis-ANS in the presence of BHA in its low-pH conformation is twenty-fold higher than in the presence of BHA in its neutral-pH conformation. The use of this probe provides a sensitive method for investigating the kinetics of the irreversible conformational change of BHA induced by low pH. At pH5.0 the reaction is described by a rapid burst followed by a double exponential increase in the fluorescence of bis-ANS, with rate constants of 5.2 +/- 0.9 x 10(-3) sec-1 and 6.7 +/- 1.9 x 10(-4) sec-1. This reaction is sensitive to the presence of tert-butylhydroquinone, an inhibitor of the conformational transition of BHA. The dependence of the reaction rate on pH indicates that the acid-induced conformational change is dependent upon the multiple protonation of the neutral-pH conformation of BHA.
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Authors | R C Bethell, N M Gray, C R Penn |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 206
Issue 1
Pg. 355-61
(Jan 05 1995)
ISSN: 0006-291X [Print] United States |
PMID | 7818541
(Publication Type: Journal Article)
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Chemical References |
- Anilino Naphthalenesulfonates
- Antioxidants
- Fluorescent Dyes
- Hemagglutinin Glycoproteins, Influenza Virus
- Hemagglutinins, Viral
- Hydroquinones
- Peptide Fragments
- Viral Envelope Proteins
- 5,5'-bis(8-(phenylamino)-1-naphthalenesulfonate)
- Bromelains
- 2-tert-butylhydroquinone
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Topics |
- Anilino Naphthalenesulfonates
- Antioxidants
(pharmacology)
- Bromelains
- Fluorescent Dyes
- Hemagglutinin Glycoproteins, Influenza Virus
- Hemagglutinins, Viral
(chemistry)
- Hydrogen-Ion Concentration
- Hydroquinones
(pharmacology)
- Kinetics
- Peptide Fragments
(chemistry)
- Protein Conformation
(drug effects)
- Spectrometry, Fluorescence
- Time Factors
- Viral Envelope Proteins
(chemistry)
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