[Detection of T-cell receptor gamma chain V gene rearrangements in angioimmunoblastic lymphadenopathy type T-cell lymphoma by PCR and family specific primers].

Abstract

Using PCR method and family specific primers for T-cell receptor gamma chain V gene, the T-cell colonalities of 12 cases of AILD-TCL were analyzed. The results showed monoclonal DNA amplification patterns of TCR gamma in all 12 cases (13 specimens), whereas the DNA from normal peripheral lymphocytes and 2 samples of atypical lymphoid hyperplasia showed polyclonal rearrangement patterns. Our results suggest that AILD-TCL is a monoclonal proliferative disorder originating from T-cell. The PCR method and family specific primers could successfully amplicate the template DNA from paraffin sections, so it is a sensitive, simple and rapid method for routine hematopathologic studies.

• Authors: G D Li, J Lorenzen, M L Hansmann
• Journal: Zhonghua bing li xue za zhi = Chinese journal of pathology (Zhonghua Bing Li Xue Za Zhi) Vol. 23 Issue 4 Pg. 211-4 (Aug 1994) ISSN: 0529-5807 [Print] China
• PMID: 7805148 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Neoplasm

Topics

• Adult
• Aged
• Base Sequence
• DNA, Neoplasm (genetics)
• Female
• Follow-Up Studies
• Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor
• Humans
• Immunoblastic Lymphadenopathy (genetics)
• Lymphoma, T-Cell (genetics)
• Male
• Middle Aged
• Molecular Sequence Data
• Polymerase Chain Reaction
• Prognosis