Recombinant bifunctional molecule FV/IFN-gamma possesses the anti-tumor FV as well as the gamma interferon activities.

Abstract

Recombinant DNA techniques were used to clone, construct and express the bifunctional molecule FV/IFN-gamma. The FV/IFN-gamma is a single-chain 42KD fusion protein expressed in E. coli under control of the strong T7 bacteriophage promoter in the expression vector pT7-7-FV-IFN-gamma. The fused gene fragment FV-IFN-gamma containing a single-chain anti-TAG72 FV gene fragment as well as the human recombinant cDNA fragment of IFN-gamma molecule. The renatured soluble form of FV/IFN-gamma was purified from E. coli inclusion bodies using HTPT chromatography. The yield of this fusion protein was estimated at 10mg/L. Our data showed that the FV/IFN-gamma molecule retained the TAG72 antigen-binding specificity and the IFN-gamma activity as measured in ELISA, Western blotting and up-regulation of CEA expression by IFN-gamma. Therefore, it may prove to be useful in targeting the biological effect of IFN-gamma to tumor cells and stimulating its immune destruction.

• Authors: J Xiang, Y Qi, X Luo, E Liu
• Journal: Cancer biotherapy (Cancer Biother) Vol. 8 Issue 4 Pg. 327-37 ( 1993) ISSN: 1062-8401 [Print] United States
• PMID: 7804374 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Carcinoembryonic Antigen
• Fv-interferon gamma
• Glycoproteins
• Immunoglobulin Fragments
• Recombinant Fusion Proteins
• Recombinant Proteins
• immunoglobulin Fv
• tumor-associated antigen 72
• Interferon-gamma

Topics

• Antigens, Neoplasm (immunology)
• Base Sequence
• Carcinoembryonic Antigen (analysis)
• Escherichia coli (genetics)
• Genetic Vectors
• Glycoproteins (immunology)
• Humans
• Immunoglobulin Fragments (biosynthesis, chemistry, genetics, pharmacology)
• Interferon-gamma (biosynthesis, chemistry, genetics, pharmacology)
• Molecular Sequence Data
• Recombinant Fusion Proteins (biosynthesis, chemistry, genetics, pharmacology)
• Recombinant Proteins
• Tumor Cells, Cultured