Hyperthermia enhances the cytotoxicity against hypoxic cells of RP-170, a new 2-nitroimidazole nucleoside hypoxic cell sensitizer.

Abstract

The effect of the new hypoxic cell sensitizer, 1-[2-hydroxy-1-(hydroxymethyl)-ethoxy]methyl-2-nitroimidazole (RP-170), combined with heat against EMT6/KU cells, was determined under conditions of in vitro hypoxia. Heat-induced cytotoxicity for the EMT6/KU cells was increased to a greater extent under conditions of hypoxia and a normal pH of the medium. Hypoxia also reduced the surviving fraction of the cells treated either with RP-170 alone or with RP-170 plus heat. The concomitant treatment of RP-170 and heat inhibited the clonogenic activity of the EMT6/KU cells under conditions of in vitro hypoxia in all experimental groups, with a significant difference (p < 0.05). Therefore, RP-170 combined with exposure to heat may be an effective treatment for hypoxic cells in a solid tumor, as these cells are resistant to radiation and/or to many chemotherapeutic agents.

• Authors: Y Emi, Y Maehara, T Kusumoto, H Baba, M Sakaguchi, K Sugimachi
• Journal: Oncology (Oncology) 1995 Jan-Feb Vol. 52 Issue 1 Pg. 55-9 ISSN: 0030-2414 [Print] Switzerland
• PMID: 7800343 (Publication Type: Journal Article)

Chemical References

• Nitroimidazoles
• Nucleosides
• Radiation-Sensitizing Agents
• RP 170
• Misonidazole

Topics

• Animals
• Cell Hypoxia
• Cell Survival (drug effects)
• Female
• Hot Temperature
• Mice
• Mice, Inbred BALB C
• Misonidazole (pharmacology)
• Nitroimidazoles (pharmacology)
• Nucleosides (pharmacology)
• Radiation-Sensitizing Agents (pharmacology)
• Tumor Cells, Cultured