19-Acetylenic-deoxycorticosterone inhibits 19-hydroxylase and 11 beta-hydroxylase activities in dispersed bovine zona fasciculata cells.

Abstract

19-Acetylenic-deoxycorticosterone (19-A-DOC) is believed to be a competitive irreversible inhibitor of the synthesis of 19-nor-deoxycorticosterone (19-nor-DOC), a potent mineralocorticoid implicated in some forms of human and animal hypertension. It has been shown to inactivate 11 beta/19-hydroxylase in hamster adrenal mitochondria. Dispersed bovine zona fasciculata cells were incubated for one hour with 1.5 x 10(-8) M ACTH and 0, 1, 10, or 100 microM 19-A-DOC and tritiated deoxycorticosterone (DOC) substrate. Steroids were separated using two sequential thin-layer chromatography systems and their tritium content was counted and corrected for recovery. The 19-A-DOC decreased synthesis of 19-hydroxydeoxycorticosterone, the precursor of 19-nor-DOC. The inhibitor also impaired 11-hydroxylation of DOC to form corticosterone. The data suggest that 19-A-DOC is an effective inhibitor of 11 beta/19-hydroxylase activity in dispersed bovine adrenal cells.

• Authors: M L Delaney, J C Melby
• Journal: Steroids (Steroids) Vol. 60 Issue 3 Pg. 265-7 (Mar 1995) ISSN: 0039-128X [Print] United States
• PMID: 7792829 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Tritium
• 19-acetylenic-deoxycorticosterone
• Desoxycorticosterone
• Adrenocorticotropic Hormone
• Steroid Hydroxylases
• steroid 19-hydroxylase
• Steroid 11-beta-Hydroxylase

Topics

• Adrenocorticotropic Hormone (pharmacology)
• Animals
• Cattle
• Desoxycorticosterone (administration & dosage, analogs & derivatives, metabolism, pharmacology)
• Female
• Steroid 11-beta-Hydroxylase (antagonists & inhibitors)
• Steroid Hydroxylases (antagonists & inhibitors)
• Tritium
• Zona Fasciculata (enzymology)