Complementation of a Streptomyces lividans Leu- mutant by the Actinoplanes teichomyceticus leuC gene.

Abstract

A leucine auxotroph of Streptomyces lividans (Sl), designated PC196, was unable to convert alpha-isopropylmalate into the beta-isomer. A DNA fragment from Actinoplanes teichomyceticus (At) cloned into the Streptomyces vector pIJ702 complemented PC196. Sequence analysis of the 3.0-kb insert revealed one complete ORF with high similarity to other leuC genes encoding the large subunit of isopropylmalate isomerase (IPMI), and the 5' end of a second ORF corresponding to leuD, which encodes the smaller subunit of IPMI. Further subcloning established that Sl strain PC196 is defective in the large subunit of IPMI.

• Authors: P Castelli, S Donadio, F Marinelli, A Borghi, M Sosio
• Journal: Gene (Gene) Vol. 158 Issue 1 Pg. 97-100 (May 26 1995) ISSN: 0378-1119 [Print] Netherlands
• PMID: 7789819 (Publication Type: Journal Article)

Chemical References

• Bacterial Proteins
• Hydro-Lyases
• LeuC protein, bacteria
• isopropylmalate isomerase
• Isomerases

Topics

• Actinomycetaceae (genetics)
• Bacterial Proteins (genetics)
• Cloning, Molecular
• Genes, Bacterial
• Genetic Complementation Test
• Hydro-Lyases
• Isomerases (genetics)
• Molecular Sequence Data
• Mutation
• Streptomyces (genetics)