Preservation of acetylcholine muscarinic M2 receptor G-protein interactions in the neocortex of patients with Alzheimer's disease.

The efficacy of acetylcholine muscarinic M2 receptor-G protein coupling was investigated in Alzheimer's disease and control neocortical membranes by measuring the effects of MgCl2 and 5'-guanylylimidodiphosphate (Gpp[NH]p) on high-affinity [3H]oxotremorine-M ([3H]OXO-M) binding. MgCl2 gave similar enhancements of [3H]OXO-M binding in Alzheimer's disease and control occipital cortex. In contrast, MgCl2 enhanced [3H]OXO-M binding was significantly higher in Alzheimer's disease superior temporal cortex, compared to controls. MgCl2 enhanced [3H]OXO-M binding in both the occipital and temporal cortices of the Alzheimer's disease cases was reversed to control levels by Gpp[NH]p. It is concluded that the number of high-affinity muscarinic M2 sites is increased in Alzheimer's disease superior temporal, but not occipital, cortex and that M2 sites in both regions maintain an efficient G-protein coupling.
AuthorsA Hérnandez-Hérnandez, A Adem, R Ravid, R F Cowburn
JournalNeuroscience letters (Neurosci Lett) Vol. 186 Issue 1 Pg. 57-60 (Feb 15 1995) ISSN: 0304-3940 [Print] IRELAND
PMID7783950 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Muscarinic Antagonists
  • Receptors, Muscarinic
  • Guanylyl Imidodiphosphate
  • Oxotremorine
  • GTP-Binding Proteins
  • Aged
  • Alzheimer Disease (metabolism)
  • Cerebral Cortex (metabolism)
  • Female
  • GTP-Binding Proteins (metabolism)
  • Guanylyl Imidodiphosphate (pharmacology)
  • Humans
  • In Vitro Techniques
  • Male
  • Membranes (metabolism)
  • Muscarinic Antagonists
  • Oxotremorine (metabolism)
  • Receptors, Muscarinic (metabolism)

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