Nucleotide sequence analysis of the carbomycin biosynthetic genes including the 3-O-acyltransferase gene from Streptomyces thermotolerans.

Abstract

A 3.2-kb DNA fragment of the carbomycin biosynthetic region including the 3-O-acyltransferase gene (acyA) from Streptomyces thermotolerans was sequenced, and four ORFs were found in the fragment. The second ORF, designated ORF-A, was transcribed in the opposite direction to the other three ORFs. The first ORF was identified as carA, a gene for carbomycin resistance. The amino acid sequence of ORF-A was homologous to proteins of the cytochrome P-450 family. Streptomyces lividans transformed with pCB20, in which ORF-A was subcloned, epoxidized carbomycin B at its C-12, 13 positions, thus producing carbomycin A. The third ORF, the amino acid sequence of which showed a homology to macrolide antibiotics O-acyltransferases was identified as acyA. The last ORF (ORF-B), which starts just 3 bp downstream from the TGA termination codon of acyA, was thought to be a carbomycin 4-O-methyltransferase gene, because the amino acid sequence deduced from ORF-B showed high homology to a putative midecamycin 4-O-methyltransferase encoded on mdmC.

• Authors: A Arisawa, H Tsunekawa, K Okamura, R Okamoto
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 59 Issue 4 Pg. 582-8 (Apr 1995) ISSN: 0916-8451 [Print] England
• PMID: 7772821 (Publication Type: Journal Article)

Chemical References

• Bacterial Proteins
• DNA, Bacterial
• Leucomycins
• carbomycin
• Acetyltransferases
• carbomycin 3-O-acetyltransferase

Topics

• Acetyltransferases (genetics)
• Amino Acid Sequence
• Bacterial Proteins
• Base Sequence
• Carbohydrate Sequence
• Cloning, Molecular
• DNA, Bacterial
• Leucomycins (biosynthesis)
• Molecular Sequence Data
• Sequence Homology, Amino Acid
• Streptomyces (enzymology, genetics)