Abstract |
A simple and rapid method for extraction of seven dinitroaniline herbicides from human whole blood and urine using Sep-Pak C18 cartridges is presented. After mixing with distilled water, whole blood and urine samples containing the herbicides were loaded on Sep-Pak C18 cartridges and eluted with chloroform- methanol (9:1). The recovery of the seven compounds that were added to whole blood and urine was more than 91%. The herbicides were detected by capillary gas chromatography (GC) with electron-capture detection (ECD), surface-ionization detection ( SID), nitrogen- phosphorus detection ( NPD), and flame-ionization detection (FID). GC-ECD generally gave the highest sensitivity for all herbicides, whereas GC-FID gave the lowest sensitivity. Isopropalin could be detected by GC- SID with a sensitivity comparable with that of GC-ECD; high sensitivity could also be obtained for isopropalin, pendimethalin, and nitralin using GC- NPD. The calibration curve for the seven herbicides measured by GC-ECD showed linearity in the range of 0.047-3.0 pmol on-column. The detection limits of the herbicides were 24-45 fmol on-column for whole blood and 19-40 fmol on-column for urine.
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Authors | T Kumazawa, K Sato, H Seno, A Ishii, O Suzuki |
Journal | Journal of analytical toxicology
(J Anal Toxicol)
1995 Mar-Apr
Vol. 19
Issue 2
Pg. 95-8
ISSN: 0146-4760 [Print] England |
PMID | 7769795
(Publication Type: Journal Article)
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Chemical References |
- Aniline Compounds
- Herbicides
- Nitro Compounds
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Topics |
- Aniline Compounds
(analysis, blood, urine)
- Chromatography, Gas
(instrumentation, methods)
- Herbicides
(analysis, blood, urine)
- Humans
- Nitro Compounds
(analysis, blood, urine)
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