Abstract |
The hydrolytic metabolism of cocaine into benzoylecgonine, ecgonine methyl ester, and ecgonine was studied in the human hepatoma cell line Hep-G2 and in the nontumorigenic fetal hepatic cell line WRL-68. Also, the toxicological response of these cells to cocaine was compared to previously published results obtained with perfused liver cells and in vivo systems. Our experiments indicated that Hep-G2 appear to have similar metabolic and toxicological patterns to in vivo and perfused cell systems. The WRL-68 tissue culture system was found to be less similar. These results suggest Hep-G2 cells can be utilized to study cocaine metabolism and toxicology, and possibly in studies involving other xenobiotic compounds.
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Authors | P M Falk, R T Sabater, D D Carballo Jr |
Journal | Journal of pharmacological and toxicological methods
(J Pharmacol Toxicol Methods)
Vol. 33
Issue 2
Pg. 113-20
(Apr 1995)
ISSN: 1056-8719 [Print] United States |
PMID | 7766918
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- benzoylecgonine
- ecgonine
- Cocaine
- ecgonine methyl ester
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Topics |
- Carcinoma, Hepatocellular
- Cell Death
(drug effects)
- Cell Division
(drug effects)
- Cell Line
- Chromatography, Gas
- Cocaine
(analogs & derivatives, analysis, metabolism, toxicity)
- Humans
- Liver
(cytology, drug effects, metabolism)
- Mass Spectrometry
- Tumor Cells, Cultured
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