Characterization of leucocin B-Ta11a: a bacteriocin from Leuconostoc carnosum Ta11a isolated from meat.

Abstract

Leuconostoc (Lc.) carnosum Ta11a, isolated from vacuum-packaged processed meats, produced a bacteriocin designated leucocin B-Ta11a. The crude bacteriocin was heat stable and sensitive to proteolytic enzymes, but not to catalase, lysozyme, or chloroform. It was active against Listeria monocytogenes and several lactic acid bacteria. Leucocin B-Ta11a was optimally produced at 25 degrees C in MRS broth at an initial pH of 6.0 or 6.5. An 8.9-MDa plasmid in Leuconostoc carnosum Ta11a hybridized to a 36-mer oligonucleotide probe (JF-1) that was homologous to leucocin A-UAL187. A 4.9-kb Sau3A fragment from a partial digest of the 8.9-MDa plasmid was cloned into pUC118. The 8.1-kb recombinant plasmid (pJF8.1) was used for sequencing and revealed the presence of two open reading frames (ORFs). ORF1 codes for a protein of 61 amino acids comprising a 37-amino-acid bacteriocin that was determined to be the leucocin B-Ta11a structural gene by virtue of its homology to leucocin A-UAL 187 (Hastings et al. 1991. J. Bacteriol 173:7491-7500). The 24-amino-acid N-terminal extension, however, differs from that of leucocin A-UAL187 by seven residues. The predicted protein of the ORF2 has 113 amino acids and is identical with the amino acid sequence of the cognate ORF of the leucocin A-UAL 187 operon.

• Authors: J V Felix, M A Papathanasopoulos, A A Smith, A von Holy, J W Hastings
• Journal: Current microbiology (Curr Microbiol) Vol. 29 Issue 4 Pg. 207-12 (Oct 1994) ISSN: 0343-8651 [Print] United States
• PMID: 7765496 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacteriocins
• leucocin B-Ta11a
• mesentericin Y105 protein, Leuconostoc mesenteroides
• leucocin A-UAL 187
• Endopeptidases

Topics

• Amino Acid Sequence
• Bacteriocins (chemistry, genetics, isolation & purification)
• Base Sequence
• Chemical Phenomena
• Chemistry, Physical
• Endopeptidases (pharmacology)
• Food Contamination
• Genes, Bacterial
• Leuconostoc (chemistry, genetics, isolation & purification)
• Meat (microbiology)
• Molecular Sequence Data
• Open Reading Frames
• Plasmids (genetics)
• Sequence Alignment
• Sequence Homology, Amino Acid