Effects of microbial products on glucose consumption and morphology of macrophages.

Abstract	We studied the effects of microbial products on glucose consumption and morphology of macrophages which were elicited with thioglycollate medium. Macromolecules such as lipopolysaccharide (LPS), tumor promoters, and respiratory inhibitors increased macrophage glucose consumption without inducing evident morphological changes. The assay system was used to screen for active substances in culture broth extracts from actinomycetes. Among them, aureothin increased glucose consumption of macrophages and inhibited respiration of a rat mitochondrial fraction. Concanamycin A induced morphological changes of macrophages into needle-like shapes but not of cloned cells including the macrophage-like cells J774.1. This compound changed fibrosarcoma L929 cells into round shapes without affecting the shape of a nontransformed fibroblast, BALB/3T3 cells. Antimycin and concanamycin A increased tumor-killing activity of macrophages when added during the effector phase. These results suggest that this assay system is simple and sufficiently reproducible and thus usable for screening for modulators of macrophage function among natural products.
Authors	J Magae, K Munemura, C Ichikawa, K Osada, T Hanada, R F Tsuji, M Yamashita, A Hino, T Horiuchi, M Uramoto
Journal	Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 57 Issue 10 Pg. 1628-31 (Oct 1993) ISSN: 0916-8451 [Print] England
PMID	7764260 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Anti-Bacterial Agents Antifungal Agents Carcinogens Chromones Lipopolysaccharides Macrolides concanamycin A aureothin Glucose
Topics	3T3 Cells Animals Anti-Bacterial Agents (pharmacology) Antifungal Agents (pharmacology) Carcinogens (pharmacology) Cell Line Cell Size (drug effects) Cell Survival Chromones (pharmacology) Enzyme-Linked Immunosorbent Assay Fibrosarcoma (pathology) Glucose (metabolism) Lipopolysaccharides (pharmacology) Macrolides Macrophages (cytology, drug effects, metabolism) Mice Mice, Inbred BALB C Mitochondria, Liver (drug effects) Tumor Cells, Cultured

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