Abstract |
Tissue PIVKA-II was examined in 32 hepatocellular carcinomas and 2 metastatic liver tumors using indirect immunofluorescence, and the results were compared with the size, histological grading and serum PIVKA-II level. The specificity of this method was confirmed by the disappearance of reactivity in PLC/PRF/5 cells after the addition of vitamin K to the culture medium. Positive PIVKA-II staining was observed as a clustered or a single cell pattern only in the HCC nodules, but not in the surrounding cirrhotic tissue. PIVKA-II staining was observed in all HCC groups regardless of histological grade. There was no relationship between PIVKA-II staining and the size of HCC. PIVKA-II was detected immunohistochemically even in small HCC of patients whose plasma PIVKA-II levels were below the detection limit. These results suggest that PIVKA-II production is a specific phenotype of HCC regardless of its histological grading and demonstrate that this immunofluorescent PIVKA-II staining is more sensitive and useful than plasma PIVKA-II assay for the diagnosis of HCC.
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Authors | N Kuwahara, T Higashi, K Nouso, T Ito, T Tsuji |
Journal | Acta medica Okayama
(Acta Med Okayama)
Vol. 49
Issue 1
Pg. 19-24
(Feb 1995)
ISSN: 0386-300X [Print] Japan |
PMID | 7762405
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Biomarkers, Tumor
- Protein Precursors
- acarboxyprothrombin
- Prothrombin
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Topics |
- Biomarkers
- Biomarkers, Tumor
(analysis, blood)
- Carcinoma, Hepatocellular
(blood, chemistry, pathology)
- Fluorescent Antibody Technique
- Humans
- Immunohistochemistry
- Liver Neoplasms
(blood, chemistry, pathology)
- Neoplasm Staging
- Protein Precursors
(analysis)
- Prothrombin
(analysis)
- Tumor Cells, Cultured
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