Immunochemical detection of urinary 5-methyl-2'-deoxycytidine as a potential biologic marker for leukemia.
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| Abstract |
A monoclonal antibody against 5-methylcytidine was prepared and characterized. This antibody, termed AMC, was reactive with compounds that had 5-methylcytosine structure (i.e. 5-methyl-2'-deoxycytidine, 5-methylcytidine and 5-methylcytosine). AMC had the highest reactivity to 5-methyl-2'-deoxycytidine among reactive compounds and had no or very slight cross-reactivity to cytidine-related compounds and any other compounds. Analysis of immunoreactive materials in urine revealed that 5-methyl-2'-deoxycytidine rather than 5-methylcytidine was, contrary to our expectation, the major component. Then the inhibition ELISA system using AMC was established and urinary levels of 5-methyl-2'-deoxycytidine in healthy individuals and cancer patients were determined. The mean excretion levels of healthy individuals was 0.90 +/- 0.43 nmol/mumol creatinine and the cut-off value was set at the mean + 2 S.D. of healthy individuals (1.76 nmol/mumol creatinine). Among various types of cancer tested, elevated levels of 5-methyl-2'-deoxycytidine were detected in leukemia patients. From these results, urinary 5-methyl-2'-deoxycytidine might be applicable as a biologic marker for leukemia.
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| Authors | K Itoh, S Aida, S Ishiwata, T Yamaguchi, N Ishida, M Mizugaki |
| Journal | Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 234 Issue 1-2 Pg. 37-45 (Jan 31 1995) ISSN: 0009-8981 [Print] Netherlands |
| PMID | 7758221 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't) |
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