The ability of guinea pigs to synthesize carnitine at a normal rate from epsilon-N-trimethyllysine or gamma-butyrobetaine in vivo is not compromised by experimental vitamin C deficiency.

Abstract

Experimental vitamin C deficiency in guinea pigs is associated with low carnitine concentrations in blood and some tissues. Ascorbic acid is a cofactor for two enzymes in the pathway of carnitine biosynthesis. The effect of experimental vitamin C deficiency on the ability of guinea pigs to synthesize carnitine was in animals fed a vitamin C-deficient diet for 28 days. On days 19 to 28, supplements (0.5 mmol.kg body weight-1.d-1) of the carnitine precursors epsilon-N-trimethyllysine or gamma-butyrobetaine were administered orally. Ascorbate-supplemented, ascorbate-deficient, and pair-fed (to ascorbate-deficient) animals showed an increase in the rate of carnitine biosynthesis (as estimated from measured rates of carnitine excretion) of 32 to 40 mumol.kg body weight-1.d-1 following supplementation with epsilon-N-trimethyllysine. Likewise, animals in each experimental group showed an increase in the rate of carnitine biosynthesis of 41 to 50 mumol.kg body weight-1.d-1 after supplementation with gamma-butyrobetaine. These results indicate that scorbutic guinea pigs are able to synthesize carnitine at a normal or above-normal rate. For guinea pigs not given a carnitine precursor supplement, rates of free and total carnitine excretion for ascorbate-deficient (but not pair-fed) animals were threefold higher than for ascorbate-supplemented animals during days 19 to 28 of the feeding regimen. Thus, carnitine depletion in vitamin C deficiency likely is due to excessive urinary excretion of carnitine and not to a decreased rate of carnitine biosynthesis.

• Authors: C J Rebouche
• Journal: Metabolism: clinical and experimental (Metabolism) Vol. 44 Issue 5 Pg. 624-9 (May 1995) ISSN: 0026-0495 [Print] United States
• PMID: 7752911 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Betaine
• trimethyllysine
• gamma-butyrobetaine
• Lysine
• Carnitine

Topics

• Analysis of Variance
• Animals
• Ascorbic Acid Deficiency (metabolism)
• Betaine (analogs & derivatives, metabolism)
• Carnitine (blood, metabolism)
• Food, Fortified
• Guinea Pigs
• Lysine (analogs & derivatives, metabolism)
• Male
• Reference Values
• Time Factors
• Weight Gain