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Mechanisms of eosinophilia in mice infested with larval Haemaphysalis longicornis ticks.

Abstract
Infestation of larval Haemaphysalis longicornis ticks induced a threefold increase of eosinophils in the peripheral blood of normal WBB6F1- +/+ mice 2 days after tick infestation. In genetically mast cell-deficient WBB6F1- W/Wv mice, a threefold increase of blood eosinophils was observed 6 days after the tick infestation. However, marked infiltration of eosinophils was detected in the tick infestation sites of the WBB6F1- +/+ mice but not the WBB6F1- W/Wv mice. When the mast cell deficiency of WBB6F1- W/Wv mice had been rescued locally by intradermal injections of WBB6F1- +/+ mouse-derived cultured mast cells, a rapid increase of blood eosinophils and tissue infiltration of eosinophils were revealed following tick infestation. The intravenous (i.v.) injection of immune spleen or lymph node cells obtained from WBB6F1- +/+ mice 10 days after tick infestation led to significant eosinophilia in naive recipient mice. Treatment with anti-Thy-1.2 or anti-CD4 monoclonal antibody (mAb) and complement (C) completely abolished the eosinophilia; the early response (2 days after tick challenge) is dependent on mast cells at the feeding site, and the late response (6 days after tick challenge) is dependent on T lymphocytes. Since amplified interleukin-5 (IL-5) cDNA was detectable in the spleen cells 4 days after tick infestation, the late response might be mediated by IL-5. The infiltration of eosinophils at the feeding site of skin appeared to be dependent on mast cells.
AuthorsH Ushio, S Hirota, T Jippo, S Higuchi, K Kawamoto, Y Kitamura, H Matsuda
JournalImmunology (Immunology) Vol. 84 Issue 3 Pg. 469-75 (Mar 1995) ISSN: 0019-2805 [Print] England
PMID7751032 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Culture Media, Conditioned
  • Interleukin-3
  • Interleukin-5
  • Pokeweed Mitogens
Topics
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cells, Cultured
  • Culture Media, Conditioned
  • Eosinophilia (immunology)
  • Immunotherapy, Adoptive
  • Interleukin-3 (biosynthesis)
  • Interleukin-5 (biosynthesis)
  • Lymph Nodes (immunology)
  • Male
  • Mice
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Pokeweed Mitogens (immunology)
  • Polymerase Chain Reaction
  • Skin (immunology)
  • Spleen (immunology)
  • Tick Infestations (immunology)

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