Abstract |
The DNA sequence of the HindIII Q2 fragment near the left terminus of the capripoxvirus (KS-1 strain) genome was determined. The sequence contains two complete open reading frames (ORFs) and a part of a third. Analysis of the deduced amino acid sequence of one of these ORFs, Q2/3L, revealed that this gene has the capacity to encode a protein which is related to members of the G-protein coupled chemokine receptor subfamily, the swinepoxvirus K2R and the human cytomegalovirus US28 ORFs. It has the key structural characteristics of the G-protein-coupled receptor superfamily, e.g., seven hydrophobic regions, predicted to span the cell membrane, and the cysteine residues in the first and second extracellular loops that are implicated in formation of a disulfide bond. Southern blot analysis showed that all three species of the Capripoxvirus genus, i.e., sheep pox, goat pox, and lumpy skin disease of cattle, contain copies of this putative G-protein-coupled chemokine receptor homologue.
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Authors | J X Cao, P D Gershon, D N Black |
Journal | Virology
(Virology)
Vol. 209
Issue 1
Pg. 207-12
(May 10 1995)
ISSN: 0042-6822 [Print] United States |
PMID | 7747471
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- DNA, Viral
- Receptors, Cytokine
- Viral Proteins
- Deoxyribonuclease HindIII
- GTP-Binding Proteins
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Topics |
- Amino Acid Sequence
- Animals
- Base Sequence
- Capripoxvirus
(classification, genetics)
- Cattle
- DNA, Viral
(genetics)
- Deoxyribonuclease HindIII
- GTP-Binding Proteins
(metabolism)
- Genes, Viral
- Molecular Sequence Data
- Receptors, Cytokine
(genetics, metabolism)
- Sequence Homology, Amino Acid
- Species Specificity
- Viral Proteins
(genetics)
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