Sequence analysis of HindIII Q2 fragment of capripoxvirus reveals a putative gene encoding a G-protein-coupled chemokine receptor homologue.

Abstract

The DNA sequence of the HindIII Q2 fragment near the left terminus of the capripoxvirus (KS-1 strain) genome was determined. The sequence contains two complete open reading frames (ORFs) and a part of a third. Analysis of the deduced amino acid sequence of one of these ORFs, Q2/3L, revealed that this gene has the capacity to encode a protein which is related to members of the G-protein coupled chemokine receptor subfamily, the swinepoxvirus K2R and the human cytomegalovirus US28 ORFs. It has the key structural characteristics of the G-protein-coupled receptor superfamily, e.g., seven hydrophobic regions, predicted to span the cell membrane, and the cysteine residues in the first and second extracellular loops that are implicated in formation of a disulfide bond. Southern blot analysis showed that all three species of the Capripoxvirus genus, i.e., sheep pox, goat pox, and lumpy skin disease of cattle, contain copies of this putative G-protein-coupled chemokine receptor homologue.

• Authors: J X Cao, P D Gershon, D N Black
• Journal: Virology (Virology) Vol. 209 Issue 1 Pg. 207-12 (May 10 1995) ISSN: 0042-6822 [Print] United States
• PMID: 7747471 (Publication Type: Comparative Study, Journal Article)

Chemical References

• DNA, Viral
• Receptors, Cytokine
• Viral Proteins
• Deoxyribonuclease HindIII
• GTP-Binding Proteins

Topics

• Amino Acid Sequence
• Animals
• Base Sequence
• Capripoxvirus (classification, genetics)
• Cattle
• DNA, Viral (genetics)
• Deoxyribonuclease HindIII
• GTP-Binding Proteins (metabolism)
• Genes, Viral
• Molecular Sequence Data
• Receptors, Cytokine (genetics, metabolism)
• Sequence Homology, Amino Acid
• Species Specificity
• Viral Proteins (genetics)