Establishment of three rat soft tissue tumor cell lines with different degrees of myogenic differentiation.

Abstract

Soft tissue malignancies often show divergent differentiation, including myogenic lineage. Five rat tumors induced with 20-hydroxymethylcholanthrene (20-OH-MCA), were cultured in vitro, and three cell lines (YMC-1, YMC-2, YMC-3) were established from them. YMC-2 and -3 cells were spindle-shaped, and YMS-1 cells round and epithelioid. In confluency, YMC-3 cells formed myotubes. The nude mouse xenotransplants showed morphological features consistent with their myogenic phenotypes. Muscle-specific enzyme activities were highest in YMC-3 cells. These lines may be useful in the investigation of the myogenic differentiation of undifferentiated mesenchymal cells.

• Authors: Y Nagashima, Y Miyagi, K Yu, I Aoki, N Yoshida, T Koshino, S Watanabe, Y Kuchino, K Misugi
• Journal: Pathology, research and practice (Pathol Res Pract) Vol. 190 Issue 11 Pg. 1071-6 (Nov 1994) ISSN: 0344-0338 [Print] Germany
• PMID: 7746741 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 20-hydroxymethylcholanthrene
• Benz(a)Anthracenes
• Carcinogens

Topics

• Animals
• Benz(a)Anthracenes
• Carcinogens
• Cell Differentiation
• Mice
• Mice, Nude
• Muscles (cytology)
• Neoplasm Transplantation (immunology)
• Neoplasms, Connective Tissue (chemically induced, pathology, ultrastructure)
• Neoplasms, Muscle Tissue (chemically induced, pathology, ultrastructure)
• Rats
• Rats, Wistar
• Tumor Cells, Cultured