HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Protective effects of a recombinant N-terminal fragment of bactericidal/permeability increasing protein on endotoxic shock in conscious rabbits.

Abstract
Endotoxin (lipopolysaccharide, LPS) can induce shock, multiple organ failure, and death. A recombinant N-terminal fragment of bactericidal/permeability increasing protein, rBPI23, binds with high affinity to gram-negative bacterial LPS and neutralizes its biological activity. We sought to determine the effect of rBPI23 on LPS-induced respiratory dysfunction and cardiovascular depression in conscious rabbits. Rabbits were injected with Escherichia coli O113 LPS (6 micrograms/kg) and treated with rBPI23 (2 mg/kg), vehicle, or control protein after recovery from surgery performed to implant catheters for hemodynamic assessments and intravenous injections. LPS challenge caused respiratory dysfunction including tachypnea, significant decreases in arterial O2 tension (PO2), arterial oxygen content, and an increase in alveolar-arterial O2 gradient (A-aDO2). LPS administration also resulted in profound and prolonged decreases in mean arterial blood pressure and cardiac index. Treatment with rBPI23 prevented LPS-induced respiratory dysfunction and significantly ameliorated the cardiovascular depression. 5 of 16 LPS-challenged animals died of respiratory failure and acidosis, whereas none died in the rBPI23 treated group (p = .11). The results demonstrate that rBPI23 protects animals against LPS-induced cardiopulmonary depression in endotoxic shock.
AuthorsY Lin, W S Ammons, W J Leach, A H Kung
JournalShock (Augusta, Ga.) (Shock) Vol. 2 Issue 5 Pg. 324-31 (Nov 1994) ISSN: 1073-2322 [Print] United States
PMID7743357 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • Anti-Infective Agents
  • Antimicrobial Cationic Peptides
  • Blood Glucose
  • Blood Proteins
  • Lactates
  • Lipopolysaccharides
  • Membrane Proteins
  • Peptide Fragments
  • Recombinant Proteins
  • bactericidal permeability increasing protein
  • Carbon Dioxide
  • Oxygen
Topics
  • Analysis of Variance
  • Animals
  • Anti-Infective Agents (pharmacology)
  • Antimicrobial Cationic Peptides
  • Blood Glucose (analysis)
  • Blood Pressure (drug effects)
  • Blood Proteins (pharmacology)
  • Body Temperature (drug effects)
  • Carbon Dioxide (blood)
  • Cardiac Output (drug effects)
  • Heart Rate (drug effects)
  • Hemodynamics (drug effects)
  • Lactates (blood)
  • Lipopolysaccharides (antagonists & inhibitors, toxicity)
  • Male
  • Membrane Proteins
  • Oxygen (blood)
  • Partial Pressure
  • Peptide Fragments (pharmacology)
  • Rabbits
  • Recombinant Proteins (pharmacology)
  • Respiration (drug effects)
  • Shock, Septic (blood, physiopathology, prevention & control)
  • Time Factors
  • Vascular Resistance (drug effects)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: