Abstract | BACKGROUND: Photodynamic treatment of red cells (RBCs) with phthalocyanines and red light inactivates lipid-enveloped viruses, such as vesicular stomatitis virus (VSV) and human immunodeficiency virus. To protect RBCs from photodynamic damage, type I free radical quenchers, such as mannitol, which did not affect virus inactivation, were added. STUDY DESIGN AND METHODS: RESULTS:
Trolox at a concentration of 5 mM was found to reduce potassium leakage during storage after Pc 4 and AIPcS4 photodynamic treatment of RBCs. Hemolysis during storage of RBC concentrates treated with Pc 4 or Pc 5 was drastically reduced by the addition of 5 mM Trolox prior to light exposure. At the same concentration, Trolox inhibited the reduction of negative surface charges on RBCs following Pc 4 and Pc 5 photodynamic treatment. Under these conditions, VSV inactivation by photodynamic treatment with all phthalocyanines was not affected by Trolox. In aqueous solution, Trolox formed a complex with AIPcS4, thus quenching the excited triplet state of AIPcS4 at a constant rate of 8.8 x 10(6) per M per second. CONCLUSION: These findings indicate that Trolox protects RBCs from phthalocyanine-photosensitized damage without affecting virus kill. The addition of Trolox would be beneficial for improving the quality of RBCs subjected to photodynamic treatment.
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Authors | E Ben-Hur, S Rywkin, I Rosenthal, N E Geacintov, B Horowitz |
Journal | Transfusion
(Transfusion)
Vol. 35
Issue 5
Pg. 401-6
(May 1995)
ISSN: 0041-1132 [Print] United States |
PMID | 7740611
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Chromans
- Indoles
- Isoindoles
- Organometallic Compounds
- Photosensitizing Agents
- aluminum tetrasulfophthalocyanine
- Potassium
- 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
- phthalocyanine
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Topics |
- Blood Transfusion
- Chromans
(administration & dosage, pharmacology)
- Electrochemistry
- Erythrocyte Membrane
(chemistry, drug effects)
- Erythrocytes
(drug effects, virology)
- Humans
- Indoles
(pharmacology)
- Isoindoles
- Light
- Organometallic Compounds
(pharmacology)
- Photosensitizing Agents
(pharmacology)
- Potassium
(blood)
- Spectrophotometry
- Vesicular stomatitis Indiana virus
(drug effects)
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