[Study of anti-loxosceles serum action on hemolytic and ulcero-necrotic cutaneous effects of Loxosceles laeta venom].

Abstract

The action of an anti-loxosceles serum on in vitro human red blood cell hemolysis and on the development of ulcero-necrotic lesions in rabbit skin, induced by loxosceles laeta venom, was studied. An 81 +/- 3% hemolysis was obtained after 72 h incubation of a 2.5% Rh+ red blood cell solution with the equivalent of one Loxosceles laeta venom gland. This parameter was not modified adding anti-loxosceles serum before, along with or after the venom (79.7 +/- 0.8, 77.3 +/- 2.1 and 80.7 +/- 0.7% respectively). After the intradermic injection of a minimal necrotizing venom dose in rabbits, a skin necrotic lesion appeared. This lesion did not appear if anti-loxosceles serum was injected together with the venom; if the serum was injected one hour after the venom, the resulting skin lesion was inflammatory but not necrotic. It is concluded that anti-loxosceles serum does not inhibit loxosceles venom induced hemolysis and causes a time dependent inhibition on skin necrotic lesions.

• Authors: M Bravo, I Oviedo, P Farías, H Schenone
• Journal: Revista medica de Chile (Rev Med Chil) Vol. 122 Issue 6 Pg. 625-9 (Jun 1994) ISSN: 0034-9887 [Print] Chile
• Vernacular Title: Estudio de la acción del suero antiloxoscélico sobre los efectos hemolíticos y ulcero-necróticos cutáneos producidos por el veneno de la araña Loxosceles laeta.
• PMID: 7732205 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Antivenins
• Immune Sera
• Spider Venoms

Topics

• Animals
• Antivenins (pharmacology)
• Hemolysis (drug effects)
• Immune Sera (pharmacology)
• Necrosis
• Rabbits
• Skin Ulcer (etiology)
• Spider Venoms (toxicity)
• Time Factors