Attenuation of ultraviolet radiation-induced edema and erythema with topical calmodulin and protein kinase C inhibitors.

Abstract

We treated Skh:HR1 hairless albino mice, NSA mice and hairless albino guinea pigs topically with N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W7) or trifluoperazine (TFP) before or after ultraviolet (UV) irradiation. When applied before irradiation, W7 and TFP prevented edema in Skh-1 mice and W7 prevented UV-induced edema in NSA mice in a dose-dependent manner. Preirradiation treatment with 2% W7 reduced erythema in guinea pigs by 50%. Epidermal histology of UVR-treated Skh-1 mice pretreated with W7 before UVR was similar to unirradiated mice. W7 did not reverse or prevent these UV-induced effects when applied after irradiation. Neither TFP nor W7 absorbed UV based on forward scattering absorbance spectra; we conclude that neither are physical or chemical sunscreens. These results suggest that calmodulin and/or protein kinase C-dependent events are involved in manifesting some of the effects of UV irradiation on skin.

• Authors: F A Anthony, J C Dowdy, M E Costlow
• Journal: Photodermatology, photoimmunology & photomedicine (Photodermatol Photoimmunol Photomed) Vol. 10 Issue 6 Pg. 227-34 (Dec 1994) ISSN: 0905-4383 [Print] England
• PMID: 7727278 (Publication Type: Journal Article)

Chemical References

• Calmodulin
• Sulfonamides
• Sunscreening Agents
• Trifluoperazine
• W 7
• Protein Kinase C

Topics

• Absorption
• Administration, Cutaneous
• Animals
• Calmodulin (antagonists & inhibitors)
• Dose-Response Relationship, Drug
• Edema (pathology, prevention & control)
• Erythema (pathology, prevention & control)
• Female
• Guinea Pigs
• Mice
• Mice, Hairless
• Mice, Inbred Strains
• Protein Kinase C (antagonists & inhibitors)
• Skin (drug effects, pathology, radiation effects)
• Skin Diseases (pathology, prevention & control)
• Skinfold Thickness
• Sulfonamides (administration & dosage, therapeutic use)
• Sunscreening Agents (therapeutic use)
• Trifluoperazine (administration & dosage, therapeutic use)
• Ultraviolet Rays (adverse effects)