Abstract |
In the present study, the effects of VIP on the growth of two human pancreatic carcinoma cell lines PU-PAN-1 and PANC-1 were determined using tritiated thymidine incorporation. VIP receptors, intracellular cAMP and polyamines were investigated. The results indicated that VIP at a concentration of 10(-8) mol/L to 10(-7) mol/L can significantly stimulate the growth of PU-PAN-1 cells but not PANC-1 cells. This effect is dose-dependent and abolished by VIP receptor antagonist, [4-C1-Phe6, Leu17] VIP, suggesting VIP receptors in PU-PAN-1 cells may mediate this effect. VIP can markedly elevate the levels of intracellular cAMP and polyamines in PU-PAN-1 cells, indicating that the growth-promoting effect stimulated by VIP may be via a rapid increase in the biosyntheses of cAMP and polyamines. In addition, the VIP-antibody inhibited the growth of PU-PAN-1 cells in serum-free culture medium. The results above suggested that VIP has an autocrine regulatory effect on this pancreatic carcinoma cell line (PU-PAN-1).
|
Authors | Y Chen, Q Chen, G Lu, Z Fan, S Zhong |
Journal | Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih
(Chin Med Sci J)
Vol. 9
Issue 4
Pg. 215-9
(Dec 1994)
ISSN: 1001-9294 [Print] China |
PMID | 7718859
(Publication Type: Journal Article)
|
Chemical References |
- Biogenic Polyamines
- Immune Sera
- Receptors, Vasoactive Intestinal Peptide
- vasoactive intestinal peptide, 4-chloro-Phe(6)-Leu(17)-
- Vasoactive Intestinal Peptide
- Cyclic AMP
|
Topics |
- Biogenic Polyamines
(biosynthesis)
- Cell Division
(drug effects)
- Cyclic AMP
(biosynthesis)
- Dose-Response Relationship, Drug
- Humans
- Immune Sera
(pharmacology)
- Pancreatic Neoplasms
(pathology)
- Receptors, Vasoactive Intestinal Peptide
(metabolism)
- Tumor Cells, Cultured
(drug effects, metabolism)
- Vasoactive Intestinal Peptide
(analogs & derivatives, antagonists & inhibitors, metabolism, pharmacology)
|