Subchromosomal band interval mapping and ordering of DNA markers in the region 3q26.3-q27 involved in the dup(3q) syndrome.

Abstract

The duplication 3q syndrome is characterized by the partial trisomy of a segment of the long arm of chromosome 3. This segment, although variable in size, includes 3q26.3-q27 as the minimal region of overlap. We have previously used patient chromosome breakpoints to select cosmids within this region. In this report, we have used two- and three-color fluorescence in situ hybridization on metaphase and interphase chromosomes to perform high-resolution cytological mapping of the six cosmids identified. The results allowed us to determine the centromere-telomere orientation, the order, and the relative distances of the markers used. Because some of the markers used are part of the consensus chromosome 3 map, our data can be easily integrated with existing mapping information about this chromosome. Our data provide a framework for further physical mapping studies of this region.

• Authors: P Rizzu, A Baldini
• Journal: Genomics (Genomics) Vol. 24 Issue 3 Pg. 580-2 (Dec 1994) ISSN: 0888-7543 [Print] United States
• PMID: 7713511 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Genetic Markers
• DNA

Topics

• Abnormalities, Multiple (genetics)
• Chromosome Aberrations
• Chromosome Banding
• Chromosome Mapping
• Chromosomes, Human, Pair 3 (ultrastructure)
• Cosmids
• DNA (genetics)
• De Lange Syndrome (genetics)
• Genetic Markers
• Humans
• In Situ Hybridization, Fluorescence
• Interphase (genetics)
• Male
• Metaphase (genetics)
• Syndrome