Protection of dogs from Lyme disease with a vaccine containing outer surface protein (Osp) A, OspB, and the saponin adjuvant QS21.

Abstract

A vaccine consisting of purified Escherichia coli-expressed recombinant full-length outer surface proteins A (OspA) and B (OspB) and the saponin adjuvant QS21 was evaluated for protection against Borrelia burgdorferi infection. Eleven beagles were vaccinated twice and then challenged with 10 field-collected adult female Ixodes scapularis. Xenodiagnosis revealed that all 11 nonvaccinated control dogs and 2 of 10 vaccinated dogs were infected with B. burgdorferi. Six of 11 control dogs also developed fever (0.75 +/- 0.38 degrees C) and were lethargic. One of the control dogs also developed a limp. Both of the infected vaccinated dogs were asymptomatic. Thus, the vaccine prevented tick-vectored infection and associated symptoms of Lyme disease.

• Authors: R T Coughlin, D Fish, T N Mather, J Ma, C Pavia, P Bulger
• Journal: The Journal of infectious diseases (J Infect Dis) Vol. 171 Issue 4 Pg. 1049-52 (Apr 1995) ISSN: 0022-1899 [Print] United States
• PMID: 7706788 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Antibodies, Bacterial
• Antigens, Bacterial
• Antigens, Surface
• Bacterial Outer Membrane Proteins
• Bacterial Vaccines
• Immunoglobulin G
• Lipoproteins
• OspA protein
• Saponins
• Vaccines, Synthetic
• OspB protein, Borrelia burgdorferi
• saponin QA-21V1

Topics

• Adjuvants, Immunologic
• Animals
• Antibodies, Bacterial (biosynthesis, blood)
• Antigens, Bacterial
• Antigens, Surface (immunology)
• Bacterial Outer Membrane Proteins (immunology)
• Bacterial Vaccines (immunology)
• Borrelia burgdorferi Group (immunology, isolation & purification)
• Dogs
• Female
• Immunoglobulin G (biosynthesis, blood)
• Lipoproteins
• Lyme Disease (prevention & control)
• Male
• Saponins (immunology)
• Ticks (microbiology)
• Vaccination
• Vaccines, Synthetic (immunology)