An angiogenic factor from human bladder cancer: the investigation of purification and biological activities.

Abstract

An angiogenic factor from human transitional cell cancer of bladder has been purified by protein extraction, cation exchange chromatography, gel filtration high-performance liquid chromatography (GE-HPLC) and reversed-phase high-performance liquid chromatography (RP-HPLC). The purified substance was named bladder cancer angiogenic factor (BCAF). Biological activity of the BCAF was assessed by the method of chick embryo chorioallantoic membrane (CAM) assay and 3H-TdR incorporation into DNA in Balb/C 3T3 cells. The BCAF displayed the potent activities of neovascularization in CAM and DNA synthesis in Balb/C 3T3 cells. The ultrastructural features of blood vessels induced by the BCAF were similar to the blood vessels in tumors. The BCAF contained a protein with an approximate molecular weight of 15,000 D which was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and silver staining. Amino acid compositions of the BCAF were also analysed by acid hydrolysis.

• Authors: B Q Chen, M S Yu, G X Shao, B F Chen
• Journal: Chinese medical journal (Chin Med J (Engl)) Vol. 106 Issue 7 Pg. 489-93 (Jul 1993) ISSN: 0366-6999 [Print] China
• PMID: 7694825 (Publication Type: Journal Article)

Chemical References

• Angiogenesis Inducing Agents
• DNA

Topics

• 3T3 Cells
• Angiogenesis Inducing Agents (isolation & purification, pharmacology)
• Animals
• Carcinoma, Transitional Cell (chemistry)
• Chick Embryo
• DNA (biosynthesis)
• Humans
• Mice
• Mice, Inbred BALB C
• Neovascularization, Pathologic
• Urinary Bladder Neoplasms (chemistry)