Induction of chromosomal aberrations, sister-chromatid exchanges and cell-cycle delay in cow peripheral blood lymphocytes treatment with two N-nitroso compounds in vitro.

Abstract

We investigated the effect of NDMA and DNSGU on the induction of chromosomal aberrations and sister-chromatid exchanges (SCEs), as well as the influence of the former compound on cell-cycle kinetics in cultured cow peripheral lymphocytes. A clastogenic effect was observed in treated cell cultures at 6 or 12 x 10(-5) M concentrations of NMDA and DNSGU, respectively, but no increase of chromosomal breaks was seen at the lowest dose. NDMA at 6 x 10(-4) M was toxic to cow lymphocytes. NDMA and DNSGU induced statistical increases of SCEs at the test doses (6 or 12 x 10(-6) and 6 or 12 x 10(-5) M, respectively). In addition, treatment with NMDA at a dose of 6 x 10(-5) M revealed significant heterogeneity of the first, second and third metaphases between treated and untreated groups. A reduction of the proliferation index and proliferation delay per cycle was shown too.

• Authors: K Siviková, J Dianovský
• Journal: Mutation research (Mutat Res) Vol. 290 Issue 2 Pg. 311-5 (Dec 1993) ISSN: 0027-5107 [Print] Netherlands
• PMID: 7694123 (Publication Type: Journal Article)

Chemical References

• Mutagens
• Nitrosourea Compounds
• Dimethylnitrosamine

Topics

• Analysis of Variance
• Animals
• Cattle
• Cell Cycle (drug effects)
• Chromosome Aberrations
• Dimethylnitrosamine (toxicity)
• Female
• Kinetics
• Lymphocytes (drug effects)
• Metaphase (drug effects)
• Mutagens (toxicity)
• Nitrosourea Compounds (toxicity)
• Sister Chromatid Exchange