In vivo CNS remyelination: HNK-1 labels newly differentiated oligodendrocytes but not precursors.

Abstract

The possibility that HNK-1 antibody might identify oligodendrocyte precursors in vivo was investigated using a combined immunocytochemical and ultrastructural analysis of remyelination in the cat optic nerve after demyelination induced by local injection of antiserum against galactocerebroside. HNK-1 antibody, in addition to antisera to galactocerebroside, glial fibrillary acidic protein, vimentin and myelin basic protein, was applied to early and late premyelinative lesions within which we have recently identified a putative precursor for the remyelinating oligodendrocyte termed the small glial cell. HNK-1 labelled oligodendrocytes in normal optic nerve and newly differentiated oligodendrocytes within lesions but did not label the small glial cells.

• Authors: W M Carroll, A R Jennings
• Journal: Journal of neurocytology (J Neurocytol) Vol. 22 Issue 8 Pg. 583-9 (Aug 1993) ISSN: 0300-4864 [Print] United States
• PMID: 7693879 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antigens, CD
• Antigens, Differentiation, T-Lymphocyte
• CD57 Antigens

Topics

• Animals
• Antibodies, Monoclonal
• Antigens, CD (immunology)
• Antigens, Differentiation, T-Lymphocyte (immunology)
• Astrocytes (physiology)
• Axons (physiology)
• CD57 Antigens
• Cats
• Cell Differentiation (immunology)
• Immunohistochemistry
• Myelin Sheath (physiology)
• Oligodendroglia (cytology, immunology)
• Optic Nerve (physiology)
• Stem Cells (immunology)