The site of antiviral action of 3-nitrosobenzamide on the infectivity process of human immunodeficiency virus in human lymphocytes.

Abstract	The C-nitroso compound 3-nitrosobenzamide, which has been shown to remove zinc from the retroviral-type zinc finger of p7NC nucleocapsid proteins, inhibits acute infection of human immunodeficiency virus type 1 in cultured human lymphocytes. The attachment of the virus to lymphocytes and the activities of critical viral enzymes, such as reverse transcriptase, protease, and integrase, are not affected by 3-nitrosobenzamide. However, the process of reverse transcription to form proviral DNA is effectively abolished by the drug, identifying the mode of action of 3-nitrosobenzamide as interrupting the role of p7NC in accurate proviral DNA synthesis during the infectious phase of the virus life cycle.
Authors	W G Rice, C A Schaeffer, L Graham, M Bu, J S McDougal, S L Orloff, F Villinger, M Young, S Oroszlan, M R Fesen
Journal	Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 90 Issue 20 Pg. 9721-4 (Oct 15 1993) ISSN: 0027-8424 [Print] United States
PMID	7692451 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Antiviral Agents Benzamides Capsid Proteins DNA, Viral Gene Products, gag NCP7 protein, Human immunodeficiency virus 1 Nitroso Compounds Viral Proteins gag Gene Products, Human Immunodeficiency Virus 3-nitrosobenzamide DNA Nucleotidyltransferases Integrases HIV Reverse Transcriptase RNA-Directed DNA Polymerase HIV Protease Zinc
Topics	Antiviral Agents Benzamides (pharmacology) Capsid Proteins DNA Nucleotidyltransferases (metabolism) DNA, Viral (biosynthesis) Gene Products, gag (chemistry) HIV Infections (drug therapy) HIV Protease (metabolism) HIV Reverse Transcriptase HIV-1 (drug effects, growth & development) Humans Integrases Nitroso Compounds (pharmacology) Proviruses (genetics) RNA-Directed DNA Polymerase (metabolism) Viral Proteins Virus Replication Zinc (chemistry) Zinc Fingers gag Gene Products, Human Immunodeficiency Virus

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!