Influence of hemoglobin variants and derivatives on glycohemoglobin determinations, as investigated by 102 laboratories using 16 methods.

Abstract

Influences of hemoglobin (Hb) variants (HbSS, HbCC, beta-thalassemia, HbAE, HbAS, HbAC, hereditary persistent HbF) and Hb derivatives (carbamylated- and acetylated-Hbs, Schiff base, and those formed in stored blood) on results of glyco-Hb assays by 102 laboratories using 16 different methods were investigated. Affinity chromatography shows deviating results only with homozygous Hb S and C. Correct interpretation of results from patients with decreased erythrocyte half-lives requires previous knowledge on this condition. Measurements of HbA1c by HPLC and electrophoresis are obviously unsuitable for homozygous hemoglobinopathies; for heterozygous hemoglobinopathies and Hb synthesis variants, HbA1c should be expressed as percentage of HbA0 + HbA1c; abnormal Hbs are usually recognized; both carbamylated- and acetylated-Hbs interfere and Schiff base must be eliminated. Except for stored blood, all Hb variants and derivatives gave erroneous results with disposable ion-exchange columns. Dako's immunoassay is not affected by Hb derivatives; glycated Hb variants are not recognized as glyco-Hb and percentages are consequently too low. Glyco-Hb by the immunoassay of Bayer (performed by one laboratory) is not affected by Hb variants and derivatives.

• Authors: C W Weykamp, T J Penders, F A Muskiet, W van der Slik
• Journal: Clinical chemistry (Clin Chem) Vol. 39 Issue 8 Pg. 1717-23 (Aug 1993) ISSN: 0009-9147 [Print] England
• PMID: 7689046 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Glycated Hemoglobin A
• Hemoglobin, Sickle
• Hemoglobins, Abnormal
• hemoglobin AC
• hemoglobin AS
• Hemoglobin C
• Hemoglobin E
• Fetal Hemoglobin

Topics

• Chemistry, Clinical (methods)
• Chromatography, Affinity
• Chromatography, High Pressure Liquid
• Electrophoresis
• Fetal Hemoglobin (analysis)
• Glycated Hemoglobin (analysis)
• Hemoglobin C (analysis)
• Hemoglobin E (analysis)
• Hemoglobin, Sickle (analysis)
• Hemoglobinopathies (blood)
• Hemoglobins, Abnormal (analysis)
• Heterozygote
• Homozygote
• Humans
• Immunoassay
• Quality Control
• Reference Values
• beta-Thalassemia (blood)